Cloning and expression of two Streptococcus mutans glucosyltransferases in Escherichia coli K-12-Reference-Cited by-同舟云学术

Cloning and expression of two Streptococcus mutans glucosyltransferases in Escherichia coli K-12

Published:1985-08 Issue:2 Volume:49 Page:414-416
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Gilpin M L,Russell R R,Morrissey P

Abstract

Chromosomal DNA from Streptococcus mutans strain MFe28 (serotype h) was cloned in the bacteriophage vector lambda L47.1. Two classes of recombinants were found which expressed glucosyltransferase activity in phage plaques: (i) gtfS, which expressed a glucosyltransferase synthesizing a water-soluble, dextranase-sensitive glucan, and (ii) gtfI, which expressed a primer-dependent glucosyltransferase synthesizing an insoluble glucan.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/iai.49.2.414-416.1985

Reference24 articles.

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