Affiliation:
1. Department of Pathobiological Sciences, University of Wisconsin-Madison, School of Veterinary Medicine, Madison, Wisconsin
Abstract
ABSTRACT
Mannheimia haemolytica
, a commensal organism of the upper respiratory tract in cattle, is the principal bacterial pathogen associated with the bovine respiratory disease complex. Adherence to the respiratory mucosa is a crucial event in its pathogenesis. However, the bacterial components that contribute to this process are not fully characterized. In this study, we demonstrated that
M. haemolytica
adhered to bovine bronchial epithelial cells (BBEC) in vitro and that adherence was inhibited by anti-
M. haemolytica
antibody. Western blot analysis of
M. haemolytica
proteins that bind to BBEC showed a dominant protein band with an apparent molecular mass of ∼30 kDa. Peptide sequences for the 30-kDa BBEC-binding proteins, as determined by liquid chromatography-tandem mass spectrometry, matched two
M. haemolytica
surface proteins: heat-modifiable outer membrane protein A (OmpA) and lipoprotein 1 (Lpp1). Western blotting showed that the 30-kDa protein band is recognized by both anti-
M. haemolytica
OmpA and anti-Lpp1 antibodies. Furthermore, incubation with anti-OmpA and anti-Lpp1 antibodies significantly inhibited
M. haemolytica
binding to BBEC monolayers. In summary, these results suggest that OmpA and Lpp1 contribute to adherence of
M. haemolytica
to bovine respiratory epithelial cells.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
39 articles.
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