Members of the PpaA/AerR Antirepressor Family Bind Cobalamin

Abstract

ABSTRACT PpaA from Rhodobacter sphaeroides is a member of a family of proteins that are thought to function as antirepressors of PpsR, a widely disseminated repressor of photosystem genes in purple photosynthetic bacteria. PpaA family members exhibit sequence similarity to a previously defined SCHIC ( s ensor c ontaining h eme i nstead of c obalamin) domain; however, the tetrapyrrole-binding specificity of PpaA family members has been unclear, as R. sphaeroides PpaA has been reported to bind heme while the Rhodobacter capsulatus homolog has been reported to bind cobalamin. In this study, we reinvestigated tetrapyrrole binding of PpaA from R. sphaeroides and show that it is not a heme-binding protein but is instead a cobalamin-binding protein. We also use bacterial two-hybrid analysis to show that PpaA is able to interact with PpsR and activate the expression of photosynthesis genes in vivo . Mutations in PpaA that cause loss of cobalamin binding also disrupt PpaA antirepressor activity in vivo . We also tested a number of PpaA homologs from other purple bacterial species and found that cobalamin binding is a conserved feature among members of this family of proteins. IMPORTANCE Cobalamin (vitamin B 12 ) has only recently been recognized as a cofactor that affects gene expression by interacting in a light-dependent manner with transcription factors. A group of related antirepressors known as the AppA/PpaA/AerR family are known to control the expression of photosynthesis genes in part by interacting with either heme or cobalamin. The specificity of which tetrapyrroles that members of this family interact with has, however, remained cloudy. In this study, we address the tetrapyrrole-binding specificity of the PpaA/AerR subgroup and establish that it preferentially binds cobalamin over heme.