Transposable element that causes mutations in a plant pathogenic Pseudomonas sp

Abstract

A 1.3-kilobase-pair DNA element, IS51, causes a loss of virulence in the plant pathogen Pseudomonas syringae pv. savastanoi. This sequence, IS51, was first discovered in a plasmid-borne iaaM locus, which together with iaaH directs the synthesis of a virulence factor, indoleacetic acid. The spontaneous insertion of IS51 in iaaM resulted in the loss of indoleacetic acid production, attenuation of virulence, and the loss of both enzyme activities coded by iaaM and iaaH. Using a cloned IS51 element as a probe, we found that numerous homologous sequences are present in different strains of this bacterium, on both chromosomal and plasmid DNAs. By artificially positioning IS51 between a tetracycline resistance gene and a promoter region, we showed that IS51 terminated transcription in Escherichia coli.