Evolutionary analysis of the 7.1-kb beta-lactamase-specifying R-plasmid of Neisseria gonorrhoeae by restriction endonucleases

Author:

Mayer L W,Robbins K E

Abstract

A physical map of restriction enzyme sites was made for the large beta-lactamase-specifying gonococcal R-plasmid (pMR0360) isolated in 1976. Single sites in the plasmid were mapped for 11 endonucleases, and multiple sites of cleavage were mapped for 17 other enzymes. Conclusions about the structure and origin of this plasmid are discussed.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference21 articles.

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3. Determination of fragment order through partial digests and multiple enzyme digests;Dnna K. J.;Methods Enzymol.,1980

4. Davis R. W. D. Bosteln and J. R. Roth. 1980. Advanced bacterial genetics: a manual for genetic engineering p. 148-158. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.

5. A paucity of palindromes in +X174;J. Theor. Biol.,1981

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