Tn FLX : a Third-Generation mariner -Based Transposon System for Bacillus subtilis-Reference-Cited by-同舟云学术

Tn FLX : a Third-Generation mariner -Based Transposon System for Bacillus subtilis

Published:2020-05-05 Issue:10 Volume:86 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Dempwolff Felix¹^ORCID,Sanchez Sandra¹,Kearns Daniel B.¹

Affiliation:

1. Department of Biology, Indiana University, Bloomington, Indiana, USA

Abstract

The stability of transposase-encoding vectors during cloning and propagation is crucial for the reliable application of transposons. Here, we increased the stability of the mariner delivery vehicle in E. coli . Moreover, the Tn FLX transposon system will improve the application of forward genetic methods with an increased number of antibiotic resistance markers and the ability to generate unbiased green fluorescent protein (GFP) fusions to report on protein translation and subcellular localization.

Funder

Foundation for the National Institutes of Health

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02893-19

Reference79 articles.

1. A purified mariner transposase is sufficient to mediate transposition in vitro.

2. In Vivo Random Mutagenesis of Bacillus subtilis by Use of TnYLB-1, a mariner -Based Transposon

3. New transposon delivery plasmids for insertional mutagenesis in Bacillus anthracis