Influenza A Neuraminidase Antibody Assay with Sensitized Erythrocytes-Reference-Cited by-同舟云学术

Influenza A Neuraminidase Antibody Assay with Sensitized Erythrocytes

Published:1973-01 Issue:1 Volume:25 Page:97-102
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Holston J. L.¹,Dowdle W. R.¹

Affiliation:

1. Respiratory Virology Unit, Center for Disease Control, Health Services and Mental Health Administration, U.S. Department of Health, Education, and Welfare, Atlanta, Georgia 30333

Abstract

Erythrocytes sensitized with purified neuraminidase (Hong Kong) antigens were used for assay of influenza A neuraminidase antibodies. The neuraminidase indirect hemagglutination test was equal to the neuraminidase hemagglutination-inhibition (enhancement) test and appeared to be better than the neuraminidase inhibition test for detection of fourfold or greater antibody rises in paired sera from influenza patients or vaccinees. It was better than both tests for detection of neuraminidase antibody. The neuraminidase indirect hemagglutination test is simple to perform and has the advantage of direct antigen-antibody assay.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.25.1.97-102.1973

Reference16 articles.

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1. Evaluation of the Single Radial Hemolysis Test for Measuring Hemagglutinin- and Neuraminidase-Specific Antibodies to H3N2 Influenza Strains and Antibodies to Influenza B;Journal of Clinical Microbiology;1977-03

2. Measurement of antibody to influenza virus neuraminidase by single radial hemolysis in agarose gels;Infection and Immunity;1976-01

3. Preparation of Stable Sensitized Erythrocytes for Detection of Chlamydial Antibodies;Journal of Clinical Microbiology;1975-01

4. Inhibition of Virus Release by Antibodies to Surface Antigens of Influenza Viruses;Journal of Virology;1974-02