Sedimentation Changes of L Cells in a Density Gradient Early After Infection with Vaccinia Virus-Reference-Cited by-同舟云学术

Sedimentation Changes of L Cells in a Density Gradient Early After Infection with Vaccinia Virus

Published:1973-09 Issue:3 Volume:12 Page:588-593
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Ball Frederick R.¹,Medzon Edward L.¹

Affiliation:

1. Department of Bacteriology and Immunology, Health Sciences Center, University of Western Ontario, London, Ontario N6A 3K7, Canada

Abstract

By 2 h postinfection, LM cells infected with vaccinia virus show a shift in their distribution when separated on a Ficoll density gradient. This shift is dependent on both time and the multiplicity of infection and is due, at least in part, to an increase in cell size. Those cells which do shift in position in the gradient represent infected members of the population. Physical changes induced in virus-infected cells can be utilized for studying early events in virus replication.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.12.3.588-593.1973

Reference19 articles.

1. The use of Ficoll gradient centrifugation to produce synchronous mouse Iymphoma cells;Ayad S. R.;Biochem. Biophys. Res. Commun.,1969

2. The resolution of mixtures of viable mammalian cells into homogeneous fractions by zonal centrifugation;Boone C. W.;J. Cell Biol.,1968

3. The initiation of vaccinia infection;Cairns J.;Virology,1960

4. Relationship between virus-induced cellular deoxyribonucleic acid synthesis and transformation by simian virus 40;Fox T. O.;J. Virol.,1971

5. Vaccinia virus infection of synchronized pig kidney cells;Groyon R. M.;J. Virol.,1967

Cited by 3 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Monovalent cation metabolism and cytopathic effects of poliovirus-infected HeLa cells;Journal of Virology;1981-01

2. Evidence for an “Early Early” Vaccinia Virus-Induced Protein Which Causes a Density Change of Infected L-M Cells;Journal of Virology;1976-01

3. Reproduction of Poxviruses;Reproduction;1974