Affiliation:
1. U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21701
Abstract
A rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A is described. The assay procedure requires 3 to 4 h for completion by using a competitive inhibition system in which the antibody is attached to bromacetyl cellulose particles. It is accurate to a level of 0.01 μg of enterotoxin A/ml in a variety of media such as ham, milk products, crab meat, custard, etc. No significant interference was found with any media or food product tested.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference10 articles.
1. Bergdoll M. S. 1970. Enterotoxins p. 265. In T. C. Montic S. Kadis and S. J. AjI (ed.) Microbial toxins vol. 3. Academic Press Inc. New York.
2. Detection of staphylococcal enterotoxin in food;Casman E. P.;Appl. Microbiol.,1965
3. A rapid solid phase radioimmunoassay for staphylococcal B enterotoxin;Collins W. S.;J. Immunol.,1972
4. The preparation of "I'l-labeled human growth hormone of high specific radioactivity;Greenwood F. C.;Biochem. J.,1963
5. Iodine-131 labeling of purified microbial and antigens by microdiffusion;Gruber J.;Proc. Soc. Exp. Biol. Med.,1967
Cited by
13 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献