Rapid Solid-Phase Radioimmunoassay for Staphylococcal Enterotoxin A

Author:

Collins William S.1,Johnson Anna D.1,Metzger Joseph F.1,Bennett Reginald W.1

Affiliation:

1. U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21701

Abstract

A rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A is described. The assay procedure requires 3 to 4 h for completion by using a competitive inhibition system in which the antibody is attached to bromacetyl cellulose particles. It is accurate to a level of 0.01 μg of enterotoxin A/ml in a variety of media such as ham, milk products, crab meat, custard, etc. No significant interference was found with any media or food product tested.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference10 articles.

1. Bergdoll M. S. 1970. Enterotoxins p. 265. In T. C. Montic S. Kadis and S. J. AjI (ed.) Microbial toxins vol. 3. Academic Press Inc. New York.

2. Detection of staphylococcal enterotoxin in food;Casman E. P.;Appl. Microbiol.,1965

3. A rapid solid phase radioimmunoassay for staphylococcal B enterotoxin;Collins W. S.;J. Immunol.,1972

4. The preparation of "I'l-labeled human growth hormone of high specific radioactivity;Greenwood F. C.;Biochem. J.,1963

5. Iodine-131 labeling of purified microbial and antigens by microdiffusion;Gruber J.;Proc. Soc. Exp. Biol. Med.,1967

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