Development of Loop-Mediated Isothermal Amplification Assay for Detection of Entamoeba histolytica-Reference-Cited by-同舟云学术

Development of Loop-Mediated Isothermal Amplification Assay for Detection of Entamoeba histolytica

Published:2009-06 Issue:6 Volume:47 Page:1892-1895
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Liang Shih-Yu¹,Chan Yun-Hsien²,Hsia Kan-Tai¹,Lee Jing-Lun²,Kuo Ming-Chu²,Hwa Kuo-Yuan³,Chan Chi-Wen²,Chiang Ting-Yi²,Chen Jung-Sheng²,Wu Fang-Tzy²,Ji Dar-Der²

Affiliation:

1. Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Taipei, Taiwan

2. Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan

3. Department of Molecular Science and Engineering, National Taipei University of Technology, Taipei, Taiwan

Abstract

ABSTRACT A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica , one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay. No DNA was amplified from other diarrheal pathogens, such as other Entamoeba species, bacteria, and viruses. These results indicate that LAMP is a rapid, simple, and valuable diagnostic tool for epidemiological studies of amebiasis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00105-09

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