PCR Method To Identify Salmonella enterica Serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the Blood of Patients with Clinical Enteric Fever-Reference-Cited by-同舟云学术

PCR Method To Identify Salmonella enterica Serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the Blood of Patients with Clinical Enteric Fever

Published:2008-05 Issue:5 Volume:46 Page:1861-1866
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Levy Haim¹²,Diallo Souleymane³,Tennant Sharon M.¹,Livio Sofie¹,Sow Samba O.³,Tapia Milagritos¹³,Fields Patricia I.⁴,Mikoleit Matthew⁴,Tamboura Boubou³,Kotloff Karen L.¹,Lagos Rosanna⁵,Nataro James P.¹,Galen James E.¹,Levine Myron M.¹

Affiliation:

1. Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland 21201

2. Israel Institute for Biological Research, Ness Ziona, Israel

3. Centre pour le Développement des Vaccins, Bamako, Mali

4. National Salmonella Reference Laboratory, Centers for Disease Control and Prevention, Atlanta, Georgia 30333

5. Centro para Vacunas en Desarrollo, Hospital de Niños Roberto del Rio, Servicio de Salud Metropolitano Norte, Santiago, Chile

Abstract

ABSTRACT PCR methodology was developed to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B. One multiplex PCR identifies serogroup D, A, and B and Vi-positive strains; another confirms flagellar antigen “d,” “a,” or “b.” Blinded testing of 664 Malian and Chilean Salmonella blood isolates demonstrated 100% sensitivity and specificity.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00109-08

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