Fifty Percent Tissue Culture Infective Dose Assay for Determining the Titer of Infectious Human Herpesvirus 8-Reference-Cited by-同舟云学术

Fifty Percent Tissue Culture Infective Dose Assay for Determining the Titer of Infectious Human Herpesvirus 8

Published:2013-06 Issue:6 Volume:51 Page:1931-1934
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Nadgir Sagar V.¹,Hensler Heather R.¹,Knowlton Emilee R.¹,Rinaldo Charles R.¹²,Rappocciolo Giovanna¹,Jenkins Frank J.¹²³

Affiliation:

1. Department of Infectious Diseases and Microbiology, Graduate School of Public Health

2. Department of Pathology, School of Medicine

3. University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

Abstract

ABSTRACT We have developed a human herpesvirus 8 (HHV-8) 50% tissue culture infective dose (TCID 50 ) assay using the T1H6-DC-SIGN cell line. Infection of T1H6-DC-SIGN cells with HHV-8 induces expression of β-galactosidase, which was used to determine TCID 50 levels. Validation of TCID 50 values was performed by immunofluorescence assay of HHV-8 infection of immature dendritic cells at various TCID 50 doses.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00761-13

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