Confirmatory Real-Time PCR Assay for Human Papillomavirus (HPV) Type 52 Infection in Anogenital Specimens Screened for HPV Infection with the Linear Array HPV Genotyping Test

Author:

Coutlée François123,Rouleau Danielle12,Ghattas Georges1,Hankins Catherine34,Vézina Sylvie5,Coté Pierre6,Macleod John3,de Pokomandy Alexandra1,Money Deborah7,Walmsley Sharon8,Voyer Hélène1,Brassard Paul3,Franco Eduardo3

Affiliation:

1. Laboratoire de Virologie Moléculaire, Centre de Recherche, et Départements de Microbiologie et Infectiologie, de Médecine Familiale et Gastro-entérologie, Hôpital Notre-Dame du Centre Hospitalier de l'Université de Montréal, Montréal, Québec, Canada

2. Département de Microbiologie et Immunologie, Université de Montréal, Montréal, Québec, Canada

3. Division of Cancer Epidemiology and Department of Medicine, McGill University, Montreal, Québec, Canada

4. Direction de la Santé Publique de Montréal-Centre, Institut National de Santé Publique du Québec, Montréal, Québec, Canada

5. Clinique Médicale l'Actuel, Montréal, Québec, Canada

6. Clinique Médicale du Quartier-Latin, Montréal, Québec, Canada

7. Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, British Columbia, Canada

8. Division of Clinical Investigation and Human Physiology, Toronto General Research Institute, Toronto General Hospital, University of Toronto, Toronto, Ontario, Canada

Abstract

ABSTRACT A novel real-time PCR assay for detection of human papillomavirus type 52 (HPV-52) DNA (RT-52) was evaluated on 265 anogenital samples. RT-52 had a sensitivity of 98.4% and a specificity of 100% compared to conventional HPV-52 typing assays, including hybridization of PGMY products with an HPV-52-specific probe and PCR sequencing of HPV-52 E6.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference18 articles.

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