Detection of experimental Chlamydia trachomatis eye infection in conjunctival smears and in tissue culture by use of fluorescein-conjugated monoclonal antibody

Author:

Taylor H R,Agarwala N,Johnson S L

Abstract

The immunofluorescent staining of conjunctival cytology smears with an antichlamydial monoclonal antibody is a new method for the detection of chlamydial eye infection. Using a monkey model of chlamydial eye infection, we compared the monoclonal antibody staining cytology with the established methods of Giemsa-stained cytology and chlamydial culture with cycloheximide-treated McCoy cells. Compared with either culture or monoclonal antibody-stained cytology, Giemsa cytology had a low sensitivity (30 and 24%, respectively) but was highly specific (100% in each case). Compared with culture, monoclonal antibody-stained cytology was highly sensitive (94%) but appeared to lack specificity (70%). However, examination of the changes in identification rates with time suggested that monoclonal antibody-stained cytology may be even more sensitive than culture and detect lower levels of infection. This study showed that monoclonal antibody-stained cytology of conjunctival smears might be a rapid, efficient, and inexpensive method of diagnosing ocular chlamydial infection.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference31 articles.

1. Chernesky M. and J. Mahony. 1982. A tissue culture procedure for the isolation of chlamydiae from large numbers of clinical specimens p. 291-293. In P.-A. Mardh K. K. Holmes J. D. Oriel P. Piot and J. Schachter (ed.) Chlamydial infections. Elsevier Biomedical Press Amsterdam.

2. Darougar S. R. St. C. Dwyer J. D. Treharne I. A. Harper J. A. Garland and B. R. Jones. 1971. A comparison of laboratory methods of diagnosis of chlamydial infection p. 445-460. In R. L. Nichols (ed.) Trachoma and related disorders. Excerpta Medica Amsterdam.

3. Darougar S. and J. D. Treharne. 1982. Cell culture methods for the isolation of C. trachomatis-a review p. 265-274. In P.-A. Mardh K. K. Holmes J. D. Oriel P. Piot and J. Schachter (ed.) Chlanmydial infections. Elsevier Biomedical Press Amsterdam.

4. Severe endemic trachoma in Tunisia;Dawson C. R.;Br. J. Ophthalmol.,1976

5. Relative sensitivity of cell culture and yolk sac for detection of TRIC infection;Gordon F. B.;Am. J. Ophthalmol.,1967

Cited by 48 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Chlamydia Infections in Nonhuman Primates;Neglected Diseases in Monkeys;2020

2. Trachoma;Neglected Tropical Diseases;2014

3. A microbiological study of conjunctivitis with emphasis on Chlamydia trachomatis, in Northern Norway;Acta Ophthalmologica;2009-05-27

4. Trachoma;The Lancet;2008-06

5. Nucleic Acid Amplification Tests for Gonorrhea and Chlamydia: Practice and Applications;Infectious Disease Clinics of North America;2005-06

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3