Multiple Regulatory Mechanisms Act on the 5′ Untranslated Region of the S-Layer Gene from Thermus thermophilus HB8-Reference-Cited by-同舟云学术

Multiple Regulatory Mechanisms Act on the 5′ Untranslated Region of the S-Layer Gene from Thermus thermophilus HB8

Published:2001-02-15 Issue:4 Volume:183 Page:1491-1494
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Castán Pablo¹,de Pedro Miguel A.¹,Risco Cristina²,Vallés Cristina¹,Fernández Luis A.³,Schwarz Heinz⁴,Berenguer José¹

Affiliation:

1. Departamento and Centro de Biologı́a Molecular Severo Ochoa, CSIC—UAM,1 and

2. Departamento de Estructura de Macromoléculas2 and

3. Departamento de Biotecnologı́a Microbiana,3

4. Centro Nacional de Biotecnologı́a, CSIC Campus de Cantoblanco, Madrid 28049, Spain, and Max Plank Institut für Entwicklungsbiologie, D-72076 Tübingen, Germany4

Abstract

ABSTRACT The role of the 5′ untranslated region (5′UTR) of the S-layer gene from Thermus thermophilus was analyzed through the isolation of Δ5′UTR mutants. In these mutants the half-life of splA mRNA was strongly reduced and slpA transcription was no longer subjected to growth phase-dependent repression. Overproduction and detachment of the external envelopes of the mutants were observed in stationary phase.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.183.4.1491-1494.2001

Reference13 articles.

1. mRNA stabilization by the ompA 5′ untranslated region: two protective elements hinder distinct pathways for RNA degradation;Arnold T. E.;RNA,1998

2. Expression, secretion and antigenic variation of bacterial S-layer proteins;Boot H. J.;Mol. Microbiol.,1996

3. Identification of a crystalline layer on the cell envelope of the thermophilic eubacterium Thermus thermophilus;Castón J.;FEMS Lett.,1988

4. The ompA 5' untranslated RNA segment functions in Escherichia coli as a growth-rate-regulated mRNA stabilizer whose activity is unrelated to translational efficiency

5. Sequence of the S-layer gene of Thermus thermophilus HB8 and functionality of its promoter in Escherichia coli

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