Human Nasal Epithelial Cells Sustain Persistent SARS-CoV-2 Infection <i>In Vitro</i> , despite Eliciting a Prolonged Antiviral Response-Reference-Cited by-同舟云学术

Human Nasal Epithelial Cells Sustain Persistent SARS-CoV-2 Infection In Vitro , despite Eliciting a Prolonged Antiviral Response

Published:2022-02-22 Issue:1 Volume:13 Page:
ISSN:2150-7511
Container-title:mBio
language:en
Short-container-title:mBio

Author:

Gamage Akshamal M.¹,Tan Kai Sen²³⁴⁵,Chan Wharton O. Y.¹,Lew Zhe Zhang Ryan²⁴,Liu Jing²⁴,Tan Chee Wah¹,Rajagopalan Deepa⁶,Lin Quy Xiao Xuan⁶,Tan Le Min⁶,Venkatesh Prasanna Nori⁶,Ong Yew Kwang²⁷,Thong Mark²⁷,Lin Raymond Tzer Pin³⁸,Prabhakar Shyam⁶,Wang De Yun²⁴,Wang Lin-Fa¹⁹^ORCID

Affiliation:

1. Programme in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore

2. Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore

3. Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore

4. Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore, Singapore

5. Biosafety Level 3 Core Facility, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, Singapore

6. Laboratory of Systems Biology and Data Analytics, Genome Institute of Singapore, Singapore

7. Department of Otolaryngology–Head & Neck Surgery, National University Health System, Singapore

8. National Public Health Laboratory, National Centre for Infectious Diseases, Singapore

9. SingHealth Duke-NUS Global Health Institute, Singapore

Abstract

Increasing medical attention has been drawn to the persistence of symptoms (long-COVID syndrome) or live virus shedding from subsets of COVID-19 patients weeks to months after the initial onset of symptoms. In vitro approaches to model viral or symptom persistence are needed to fully dissect the complex and likely varied mechanisms underlying these clinical observations. We show that in vitro differentiated human NECs are persistently infected with SARS-CoV-2 for up to 28 dpi.

Funder

MOH | National Medical Research Council

European Allergy and Clinical Immunology Research Fellowship

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mbio.03436-21

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