Performance of Three SARS-CoV-2 Immunoassays, Three Rapid Lateral Flow Tests, and a Novel Bead-Based Affinity Surrogate Test for the Detection of SARS-CoV-2 Antibodies in Human Serum-Reference-Cited by-同舟云学术

Performance of Three SARS-CoV-2 Immunoassays, Three Rapid Lateral Flow Tests, and a Novel Bead-Based Affinity Surrogate Test for the Detection of SARS-CoV-2 Antibodies in Human Serum

Published:2021-07-19 Issue:8 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Krone Manuel¹^ORCID,Gütling Julia¹,Wagener Johannes¹²,Lâm Thiên-Trí¹,Schoen Christoph¹,Vogel Ulrich¹,Stich August³,Wedekink Florian⁴,Wischhusen Jörg⁴,Kerkau Thomas⁵,Beyersdorf Niklas⁵,Klingler Silvana⁶,Backes Simone⁵,Dölken Lars⁵,Gasteiger Georg⁶,Kurzai Oliver¹^ORCID,Schubert-Unkmeir Alexandra¹^ORCID

Affiliation:

1. Institute for Hygiene and Microbiology, University of Wuerzburg, Wuerzburg, Germany

2. Department of Clinical Microbiology, Trinity College Dublin, Dublin, Ireland

3. Department of Tropical Medicine, Missioklinik, Klinikum Wuerzburg Mitte, Wuerzburg, Germany

4. Department of Gynecology, Section for Experimental Tumor Immunology, University Hospital Wuerzburg, Wuerzburg, Germany

5. Institute for Virology and Immunobiology, University of Wuerzburg, Wuerzburg, Germany

6. Institute of Systems Immunology, University of Wuerzburg, Wuerzburg, Germany

Abstract

For the control of immunity in COVID-19 survivors and vaccinated subjects, there is an urgent need for reliable and rapid serological assays. Based on samples from 63 COVID-19 survivors up to 7 months after symptom onset, and on 50 serum samples taken before the beginning of the pandemic, we compared the performances of three commercial immunoassays for the detection of SARS-CoV-2 IgA and IgG antibodies (Euroimmun SARS-COV-2 IgA/IgG, Mikrogen recom Well SARS-CoV-2 IgA/IgG, and Serion ELISA agile SARS-CoV-2 IgA/IgG) and three rapid lateral flow (immunochromatographic) tests (Abbott PanBio COVID-19 IgG/IgM, Nadal COVID-19 IgG/IgM, and Cleartest Corona 2019-nCOV IgG/IgM) with a 50% plaque-reduction neutralization test (PRNT50) representing the gold standard.

Funder

Bundesministerium für Bildung und Forschung

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00319-21

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