Affiliation:
1. Department of Soil and Environmental Sciences, University of California, Riverside, California 92521
Abstract
Detached cowpea nodules that contained a nitrous oxide reductase-positive (Nor
+
) rhizobium strain (8A55) and a nitrous oxide reductase-negative (Nor
−
) rhizobium strain (32H1) were incubated with 1%
15
N
2
O (95 atom%
15
N) in the following three atmospheres: (i) aerobic with C
2
H
2
(10%), (ii) aerobic without C
2
H
2
, and (iii) anaerobic (argon atmosphere) without C
2
H
2
. The greatest production of
15
N
2
occurred anaerobically with 8A55, yet very little was formed with 32H1. Although acetylene reduction activity was slightly higher with 32H1, about 10 times more
15
N
2
was produced aerobically by 8A55 than by 32H1 in the absence of acetylene. The major reductive pathway of N
2
O reduction by denitrifying rhizobium strain 8A55 is by nitrous oxide reductase rather than nitrogenase.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献