Affiliation:
1. Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019
Abstract
Percoll gradient centrifugation effectively separated
Syntrophomonas wolfei
cells from
Methanospirillum hungatii
cells, resulting in a 70- to 80-fold enrichment of
S. wolfei
cells relative to
M. hungatii
cells. The separated
S. wolfei
cells were viable. Gram quantities of cellular protein which was enzymatically active and had low levels of contamination by the methanogenic cofactor, factor
420
, were obtained.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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