Affiliation:
1. Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, California, USA
Abstract
Magnetotactic bacteria (MTB) are a group of organisms that form intracellular nanometer-scale magnetic crystals though a complex process involving lipid and protein scaffolds. These magnetic crystals and their lipid membranes, termed magnetosomes, are model systems for studying bacterial cell biology and biomineralization and are potential platforms for biotechnological applications. Due to a lack of genetic tools and unculturable representatives, the mechanisms of magnetosome formation in phylogenetically deeply branching MTB remain unknown. These MTB contain elongated bullet-/tooth-shaped magnetite and greigite crystals that likely form in a manner distinct from that of the cubooctahedral-shaped magnetite crystals of the genetically tractable MTB within the
Alphaproteobacteria
. Here, we present a method for genome editing in
Desulfovibrio magneticus
RS-1, a cultured representative of the deeply branching MTB of the class
Deltaproteobacteria
. This marks a crucial step in developing
D. magneticus
as a model for studying diverse mechanisms of magnetic particle formation by MTB.
Funder
HHS | National Institutes of Health
DOD | United States Navy | Office of Naval Research
MoSTR | National Science Foundation
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
8 articles.
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