Culture-Independent Molecular Subtyping of Mycoplasma pneumoniae in Clinical Samples

Author:

Dumke Roger1,Lück Paul Christian1,Noppen Christoph2,Schaefer Christoph2,von Baum Heike3,Marre Reinhard3,Jacobs Enno1

Affiliation:

1. Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany

2. Viollier AG, Spalenring 145/147, CH-4002 Basel, Switzerland

3. Universitätsklinikum Ulm, Department of Medical Microbiology and Hygiene, Albert-Einstein-Allee 11, D-89081 Ulm, Germany

Abstract

ABSTRACT A new molecular subtyping approach was developed which is based on the amplification and sequencing of a repetitive region of the P1 gene of Mycoplasma pneumoniae . It allows the differentiation of all known subtypes and variants of M. pneumoniae as well as the identification of new subtypes directly in clinical samples to characterize endemic and epidemic M. pneumoniae infections.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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