Affiliation:
1. Praxis Biologics, Inc., Rochester, New York 14623.
Abstract
Outer membrane proteins of nontypeable (NT) Haemophilus influenzae are among the major candidates for inclusion in vaccines against these organisms. This article reports the purification of the e (P4) lipoprotein of H. influenzae and the subsequent production of antiserum directed against this protein. The anti-e polyclonal serum cross-reacted with e protein in multiple clinical NT H. influenzae isolates. Monoclonal antibody analysis of e protein showed at least one surface-exposed epitope to be conserved among NT H. influenzae strains. Anti-e serum also had bactericidal activity against multiple clinical isolates of NT H. influenzae. These results are in contrast to previous reports in the literature that purified P4 protein did not elicit biologically active antibodies. Anti-e antibodies exhibited synergistic bactericidal activity directed against NT H. influenzae when mixed with antibodies directed against another Haemophilus lipoprotein, PCP. This bactericidal synergy was observed against a variety of NT clinical isolates. We also report the cloning of the Haemophilus e lipoprotein, or hel, gene encoding the e protein and its expression and processing in Escherichia coli. The nucleotide sequence of the gene and deduced amino acid sequence of the protein are given. These results demonstrate that e protein is a viable candidate to be a component of a vaccine against NT H. influenzae.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference39 articles.
1. Protection by serum antibodies in experimental nontypable Haemophilus influenzae otitis media;Barenkamp S. J.;Infect. Immun.,1986
2. Bluestone C. D. and J. 0. Klein. 1983. Otitis media with effusion atelectasis and eustacian tube dysfunction p. 356. In C. D. Bluestone and S. E. Stool (ed.) Pediatric otolaryngology. The W. B. Saunders Co. Philadelphia.
3. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system;Bolivar F.;Gene,1977
4. Design and development of pilus vaccines for Haemophilus influenzae diseases;Brinton C. C.;Pediatr. Infect. Dis.,1988
5. Contribution of a 28-kilodalton membrane protein to the virulence of Haemophilus influenzae;Chanyangam M.;Infect. Immun.,1991
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