Search for Sendai 6/94 viral RNA in the antigen-free cell line Cl-C-2 isolated from human multiple sclerosis brain tissue

Abstract

The viral antigen-free cell line Cl-C-2, obtained from multiple sclerosis brain tissue by cell fusion with CV-1 cells, was examined for the presence of intracellular virus-specific RNA sequences of the persistent Sendai 6/94 virus by nucleic acid hybridization. As a specific probe for this assay, an in vitro synthesized cDNA was used. Oligodeoxyguanidylic acid served as a primer for the initiation of cDNA synthesis. The 6/94 RNA was detectable as expected in the viral antigen-expressing cell lines Cl-E-8 and Cl-F-2, which were used as a reference of the same source. In the viral antigen-free cell line Cl-C-2, however, no viral RNA sequences have been found by hybridization experiments. Corresponding superinfection studies confirmed the conclusion that in cell line Cl-C-2 no viral components are present. The lack of expression of viral proteins and of protection against superinfection seems to be correlated with the lack of viral RNA in Cl-C-2 cells, which may eliminate the persistent virus by a cellular defense mechanism.