Development of a Multiplex-PCR Serotyping Assay for Characterizing Legionella pneumophila Serogroups Based on the Diversity of Lipopolysaccharide Biosynthetic Loci-Reference-Cited by-同舟云学术

Development of a Multiplex-PCR Serotyping Assay for Characterizing Legionella pneumophila Serogroups Based on the Diversity of Lipopolysaccharide Biosynthetic Loci

Published:2021-10-19 Issue:11 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Nakaue Ryota¹,Qin Tian²,Morita Masatomo³,Ren Hongyu²,Chang Bin³,Murai Miyo¹⁴,Amemura-Maekawa Junko³^ORCID,Ohnishi Makoto³

Affiliation:

1. Graduate Course of Health and Social Services Master’s Program, Saitama Prefectural University, Saitama, Japan

2. National Institute for Communicable Disease Control and Prevention, and State Key Laboratory for Infectious Disease Prevention and Control, Chinese Centre for Disease Control and Prevention, Beijing, China

3. Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan

4. Department of Health Sciences, Saitama Prefectural University, Saitama, Japan

Abstract

Legionella pneumophila , which is the main cause of Legionnaires’ disease, comprises at least 15 serogroups (SGs). We show here the diversity of lipopolysaccharide biosynthetic loci among serogroups and describe the development of a PCR serotyping assay for 15 SGs based on the sequences of LPS biosynthetic loci.

Funder

Health and Labour Sciences Research Grants

Japan Agency for Medical Research and Development

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00157-21

Reference35 articles.

1. Legionnaires' Disease

2. Mode of Transmission of Legionella pneumophila