Transposition of Tn5096 from a temperature-sensitive transducible plasmid in Streptomyces spp

Author:

McHenney M A1,Baltz R H1

Affiliation:

1. Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.

Abstract

Transposon Tn5096 was inserted into a derivative of the temperature-sensitive plasmid pMT660 containing the bacteriophage FP43 pac site. The resulting plasmid, pRHB126, was transduced by FP43 into several Streptomyces species. Tn5096 transposed from pRHB126 into different sites in the genomes of Streptomyces ambofaciens, Streptomyces cinnamonensis, Streptomyces coelicolor A3(2), Streptomyces fradiae, Streptomyces griseofuscus, and Streptomyces thermotolerans.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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