Cloning and sequencing of the beta-lactam hydroxylase gene (cefF) from Streptomyces clavuligerus: gene duplication may have led to separate hydroxylase and expandase activities in the actinomycetes-Reference-Cited by-同舟云学术

Cloning and sequencing of the beta-lactam hydroxylase gene (cefF) from Streptomyces clavuligerus: gene duplication may have led to separate hydroxylase and expandase activities in the actinomycetes

Published:1991-01 Issue:1 Volume:173 Page:398-400
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Kovacevic S¹,Miller J R¹

Affiliation:

1. Department of Molecular Genetics Research, Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, Indiana 46285.

Abstract

The deacetylcephalosporin C synthetase (hydroxylase) gene from Streptomyces clavuligerus has been cloned and sequenced. The open reading frame codes for a protein with an Mr of 34,584. The hydroxylase gene (cefF) is closely linked to the epimerase gene (cefD) and the expandase gene (cefE) and is transcribed in the opposite orientation. The hydroxylase and expandase genes are 59 and 71% identical at the amino acid and DNA levels, respectively. cefE and cefF may have arisen from a gene duplication in the actinomycetes.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.173.1.398-400.1991

Reference20 articles.

1. Cloning and expression of a hybrid Streptomyces clavuligerus cefE gene in Penicillium chrysogenum;Cantwell C. A.;Curr. Genet.,1990

2. Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase from Cephalosporium acremonium;Dotzlaf J. E.;J. Bacteriol.,1987

3. Beta-lactam biosynthetic genes;Ingolia T. D.;Med. Res. Rev.,1989

4. Deacetoxycephalosporin C synthetase and deacetoxycephalosporin C hydroxylase are two separate enzymes in Streptomyces clavuligerus;Jensen S. E.;J. Antibiot.,1985

5. Kovacevic S. and J. R. Miller. Unpublished observation.

Cited by 71 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Harvesting the complex pathways of antibiotic production and resistance of soil bacilli for optimizing plant microbiome;FEMS Microbiology Ecology;2020-07-16

2. Industrial Application of 2-Oxoglutarate-Dependent Oxygenases;Catalysts;2019-02-28

3. Roles of 2-oxoglutarate oxygenases and isopenicillin N synthase in β-lactam biosynthesis;Natural Product Reports;2018

4. Unique chemistry of non-heme iron enzymes in fungal biosynthetic pathways;Natural Product Reports;2018

5. Modified Deacetylcephalosporin C Synthase for the Biotransformation of Semisynthetic Cephalosporins;Applied and Environmental Microbiology;2016-07