Evaluation of MALDI Biotyper Mycobacteria Library v3.0 for Identification of Nontuberculous Mycobacteria-Reference-Cited by-同舟云学术

Evaluation of MALDI Biotyper Mycobacteria Library v3.0 for Identification of Nontuberculous Mycobacteria

Published:2016-04 Issue:4 Volume:54 Page:1144-1147
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Rodríguez-Sánchez Belén¹²³,Ruiz-Serrano M. Jesús¹²³,Ruiz Adrián¹³,Timke Markus⁴,Kostrzewa Markus⁴,Bouza Emilio¹²³⁵

Affiliation:

1. Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Madrid, Spain

2. CIBER de Enfermedades Respiratorias (CIBERES CB06/06/0058), Madrid, Spain

3. Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain

4. Bruker Daltonik GmbH, Bremen, Germany

5. Medicine Department, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain

Abstract

ABSTRACT Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has demonstrated its ability to promptly identify nontuberculous mycobacteria using the Mycobacteria Library v2.0. However, some species are particularly difficult to identify reliably using this database, providing a low log(score). In this study, the identification power of an updated Mycobacteria Library (v3.0) has been evaluated. Overall, 109 NTM isolates were analyzed with both databases. The v3.0 database allowed a high-level confidence in the identification [log(score) value, ≥1.8] of 91.7% of the isolates versus 83.5% with the v2.0 version ( P < 0.01).

Funder

Miguel Servet Program

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02760-15

Reference7 articles.

1. Comparison of MALDI-TOF MS With HPLC and Nucleic Acid Sequencing for the Identification of Mycobacterium Species in Cultures Using Solid Medium and Broth

2. Comparison of the Bruker Biotyper and Vitek MS Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Systems for Identification of Mycobacteria Using Simplified Protein Extraction Protocols

3. Identification of Mycobacteria from Solid and Liquid Media by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry in the Clinical Laboratory