Genetic background affects the mucosal SIgA levels, parasite burden, lung inflammation and susceptibility of male mice to Ascaris suum infection.

Abstract

Ascariasis is a neglected tropical disease, widespread in the world and causing important socioeconomic impacts. The presence of various stages of worm development in the pulmonary and intestinal mucosa induces a humoral and cellular immune response. However, although there is much evidence of the protective role of mucosal immunity against various pathogens, including helminthes, there is still a gap in the knowledge about the immune response and the mechanisms of action that are involved in protection against diseases, especially in the initial phase of ascariasis. Then, the aim of this study was to evaluate the kinetic aspects of the immune parasitological parameters in intestinal and pulmonary mucosa in male mice with early ascariasis. Therefore, two mice strains showed a different susceptibility to ascariasis (BALB/c and C57BL6/j), when experimentally infected with 2,500 infective eggs of Ascaris suum from time-point 0 and the immune parasitological parameters evaluated each two days after infection, during the period of 12 days. The results were suggestive of a synergetic action of intestinal and pulmonary SIgA contributing for the protection against early ascariasis by reducing the amount of migrating larval as well as the influx of leukocytes in the lung and the consequent impair of the pulmonary capacity.