Reconciling the Potentially Irreconcilable? Genotypic and Phenotypic Amoxicillin-Clavulanate Resistance in Escherichia coli-Reference-Cited by-同舟云学术

Reconciling the Potentially Irreconcilable? Genotypic and Phenotypic Amoxicillin-Clavulanate Resistance in Escherichia coli

Published:2020-05-21 Issue:6 Volume:64 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Davies Timothy J.¹²,Stoesser Nicole¹²³^ORCID,Sheppard Anna E.¹²,Abuoun Manal⁴,Fowler Philip¹^ORCID,Swann Jeremy¹²,Quan T. Phuong¹²⁵^ORCID,Griffiths David¹⁵,Vaughan Alison¹⁵,Morgan Marcus³,Phan Hang T. T.¹,Jeffery Katie J.³,Andersson Monique³,Ellington Matt J.⁶,Ekelund Oskar⁷,Woodford Neil²⁶,Mathers Amy J.⁸^ORCID,Bonomo Robert A.⁹¹⁰,Crook Derrick W.¹²³⁵,Peto Tim E. A.¹²³⁵,Anjum Muna F.⁴,Walker A. Sarah¹²⁵

Affiliation:

1. Nuffield Department of Medicine, Oxford University, Oxford, United Kingdom

2. National Institute for Health Research Health Protection Research Unit on Healthcare Associated Infections and Antimicrobial Resistance at the University of Oxford, Oxford, United Kingdom

3. Oxford University Hospitals NHS Foundation Trust, Oxford, United Kingdom

4. Bacteriology, Animal and Plant Health Agency, Surrey, United Kingdom

5. NIHR Biomedical Research Centre, Oxford, United Kingdom

6. Antimicrobial Resistance and Healthcare Associated Infections (AMRHAI) Reference Unit, National Infection Service, Public Health England, London, United Kingdom

7. Department of Clinical Microbiology and the EUCAST Development Laboratory, Kronoberg Region, Central Hospital, Växjö, Sweden

8. Division of Infectious Diseases and International Health, Department of Medicine, Clinical Microbiology, Department of Pathology, University of Virginia Health System, Charlottesville, Virginia, USA

9. Louis Stokes Cleveland Veterans Affairs Medical Centre, Research Service, Cleveland, Ohio, USA

10. Case Western Reserve University, Departments of Medicine, Biochemistry, Molecular Biology and Microbiology, Pharmacology, and Proteomics and Bioinformatics, CWRU-Cleveland VAMC Centre for Antimicrobial Resistance and Epidemiology (Case VA CARES), and Geriatric Research Education and Clinical Centers, Louis Stokes Cleveland Department of Veterans Affairs, Cleveland, Ohio, USA

Abstract

Resistance to amoxicillin-clavulanate, a widely used beta-lactam/beta-lactamase inhibitor combination antibiotic, is rising globally, and yet susceptibility testing remains challenging. To test whether whole-genome sequencing (WGS) could provide a more reliable assessment of susceptibility than traditional methods, we predicted resistance from WGS for 976 Escherichia coli bloodstream infection isolates from Oxfordshire, United Kingdom, comparing against phenotypes from the BD Phoenix (calibrated against EUCAST guidelines).

Funder

National Institute for Health Research

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.02026-19

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