Affiliation:
1. Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9
Abstract
ABSTRACT
F plasmid TraF and TraH are required for F pilus assembly and F plasmid transfer. Using flotation sucrose density gradients, we found that TraF and TraH (as well as TraU and TraW) localized to the outer membrane in the presence of the complete F transfer region, especially TraV, the putative anchor. Mutational analysis of TraH revealed two domains that are important for its function and possible interaction with TrbI, which in turn has a role in stabilizing TraH.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology