Affiliation:
1. Biophysics Division, Indian Institute of Chemical Biology, Calcutta 700 032, India
Abstract
ABSTRACT
By using a low-resolution macrorestriction map as the foundation (R. Majumder et al., J. Bacteriol. 176:1105–1112, 1996), an ordered cloned DNA map of the 3.2-Mb chromosome of the hypertoxinogenic strain 569B of
Vibrio cholerae
has been constructed. A cosmid library the size of about 4,000 clones containing more than 120 Mb of
V. cholerae
genomic DNA (40-genome equivalent) was generated. By combining landmark analysis and chromosome walking, the cosmid clones were assembled into 13 contigs covering about 90% of the
V. cholerae
genome. A total of 92 cosmid clones were assigned to the genome and to regions defined by
Not
I,
Sfi
I, and
Ceu
I macrorestriction maps. Twenty-seven cloned genes, 9
rrn
operons, and 10 copies of a repetitive DNA sequence (IS
1004
) have been positioned on the ordered cloned DNA map.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
14 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献