Colorimetric Enzyme-Linked Immunosorbent Assay for the Identification of Strains of Rhizobium in Culture and in the Nodules of Lentils-Reference-Cited by-同舟云学术

Colorimetric Enzyme-Linked Immunosorbent Assay for the Identification of Strains of Rhizobium in Culture and in the Nodules of Lentils

Published:1979-03 Issue:3 Volume:37 Page:642-646
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Berger J. A.¹,May S. N.¹,Berger L. R.¹,Bohlool B. B.¹

Affiliation:

1. Department of Microbiology, University of Hawaii, Honolulu, Hawaii 96822

Abstract

An indirect enzyme-linked immunosorbent assay has been developed to identify strains of Rhizobium in culture and in lentil nodules. The test can be used on cells from both fresh and frozen nodules obtained from plants grown either in a growth chamber or in the field. Test results were confirmed by immunofluorescence. The enzyme-linked immunosorbent assay technique can be used for field studies and requires less antisera than other serological techniques.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.37.3.642-646.1979

Reference11 articles.

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