Powassan Viruses Spread Cell to Cell during Direct Isolation from Ixodes Ticks and Persistently Infect Human Brain Endothelial Cells and Pericytes-Reference-Cited by-同舟云学术

Powassan Viruses Spread Cell to Cell during Direct Isolation from Ixodes Ticks and Persistently Infect Human Brain Endothelial Cells and Pericytes

Published:2022-01-12 Issue:1 Volume:96 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Conde Jonas N.¹²^ORCID,Sanchez-Vicente Santiago³,Saladino Nicholas¹²,Gorbunova Elena E.¹²,Schutt William R.¹²,Mladinich Megan C.¹⁴²,Himmler Grace E.¹⁴²,Benach Jorge¹²,Kim Hwan Keun¹⁴²,Mackow Erich R.¹⁴²^ORCID

Affiliation:

1. Department of Microbiology and Immunology, Stony Brook University, Stony Brook, New York, USA

2. Center for Infectious Disease, Stony Brook University, Stony Brook, New York, USA

3. Center for Infection and Immunity, Mailman School of Public Health, Columbia University New York, New York, USA

4. Molecular and Cellular Biology Program, Stony Brook University, Stony Brook, New York, USA

Abstract

We isolated POWVs from LI deer ticks ( I. scapularis ) directly in VeroE6 cells, and sequencing revealed POWV-LI9 as a distinct lineage II POWV strain. Remarkably, inoculation of VeroE6 cells with POWV-containing tick homogenates resulted in infected cell foci in liquid culture, consistent with cell-to-cell spread.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Sterling-Turner Foundation

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01682-21

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