Immunogold silver staining for visualization of leptospires in histologic sections

Author:

Skilbeck N W,Chappel R J

Abstract

An immunogold silver stain for leptospires in sections of Formalin-fixed, paraffin-embedded tissues is described. Leptospires were intensely stained, and nonspecific staining of the tissue background was negligible.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference6 articles.

1. Improved microtechnique for the leptospiral microscopic agglutination test. Apple;Cole J. R.;Microbiol.,1973

2. Disbrey B. D. and J. H. Rack. 1970. Histological laboratory methods p. 220-223. E & S. Livingstone Edinburgh.

3. Immunogold-silver staining: new method of immunostaining with enhanced sensitivity;Holgate C. S.;J. Histochem. Cytochem.,1983

4. Differentiation of pathogenic and saprophytic leptospires. 1. Growth at low temperatures;Johnson R. C.;J. Bacteriol.,1967

5. Experimental pathogenesis of leptospiral abortion in cattle;Murphy J.C.;Am. J. Vet. Res.,1969

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