Detection of Gardnerella vaginalis in vaginal specimens by direct immunofluorescence

Author:

Hansen W1,Vray B1,Miller K1,Crokaert F1,Yourassowsky E1

Affiliation:

1. Department of Microbiology, Brugmann University Hospital, Brussels, Belgium.

Abstract

The preparation of a fluorescein-labeled Gardnerella vaginalis polyclonal antibody is described, and its usefulness is assessed for the detection of this microorganism in vaginal samples obtained from 263 women attending the gynecological department of a general hospital, 66 of whom harbored an intrauterine device. The direct immunofluorescence technique was positive for G. vaginalis in 21% of the specimens, whereas only 12.5% of the total bacteriological cultures were positive. The frequency was higher in patients harboring intrauterine devices since 34.8% exhibited positive immunofluorescence and 30.3% exhibited positive cultures.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference24 articles.

1. Detection of Gardnerella vaginalis on vaginal smears by immunofluorescence;Cano R. J.;Can. J. Microbiol.,1983

2. Haetnophilus vaginalis: vaginitis by inoculation from culture;Criswell B. S.;Obstet. Gynecol.,1969

3. Relative incidence of Cornebacterium vaginale (Haemophilus vaginalis), Neisseria gonorrhoeae, and Trichomonas spp. among women attending a venereal disease clinic;Dunkelberg W. E.;Br. J. Vener. Dis.,1970

4. Haemophilus vaginalis vaginitis: a newly defined specific infection previously classified "nonspecific vaginitis;Gardner H. L.;Am. J. Obstet. Gynecol.,1955

5. Salient features of Haemnophilus vaginalis;Greenwood J. R.;J. Clin. Microbiol.,1979

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