Affiliation:
1. Department of Entomology, University of Hawaii, Honolulu, Hawaii 96822, and Department of Entomology, Auburn University, Auburn, Alabama 368492
Abstract
Repeated exposure in the field followed by laboratory selection produced 1,800- to >6,800-fold resistance to formulations of
Bacillus thuringiensis
subsp.
kurstaki
in larvae of the diamondback moth,
Plutella xylostella.
Four toxins from
B. thuringiensis
subsp.
kurstaki
[CryIA(a), CryIA(b), CryIA(c), and CryIIA] caused significantly less mortality in resistant larvae than in susceptible larvae. Resistance to
B. thuringiensis
subsp.
kurstaki
formulations and toxins did not affect the response to CryIC toxin from
B. thuringiensis
subsp.
aizawai.
Larvae resistant to
B. thuringiensis
subsp.
kurstaki
showed threefold cross-resistance to formulations of
B. thuringiensis
subsp.
aizawai
containing CryIC and CryIA toxins. This minimal cross-resistance may be caused by resistance to CryIA toxins shared by
B. thuringiensis
subsp.
kurstaki
and
B. thuringiensis
subsp.
aizawai.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference31 articles.
1. Abbott Laboratories. 1992. B.t. products manual. Abbott Laboratories North Chicago Ill.
2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding;Bradford M. M.;Anal. Biochem.,1976
3. Bacillus thuringiensis: insects and beyond;Feitelson J. S.;Bio/Technology,1992
4. Resistance to the Bacillus thuringiensis bioinsecticide in a field population of Plutella xylostella is due to a change in a midgut membrane receptor;Ferrm J.;Proc. Natl. Acad. Sci. USA,1991
5. Fongsmut A. 1990. Diamondback moth: toxicological database resistance monitoring techniques and intraplant distribution. M.S. thesis. Oregon State University Corvallis.
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