Rotavirus G and P Genotypes in Rural Ghana

Author:

Asmah Richard H.1,Green Jon2,Armah George E.1,Gallimore Chris I.2,Gray Jim J.3,Iturriza-Gómara Mirren3,Anto Francis4,Oduro Abraham4,Binka Fred N.4,Brown David W. G.2,Cutts Felicity5

Affiliation:

1. Noguchi Memorial Institute for Medical Research, University of Ghana, Legon,1 and

2. Enteric and Respiratory Virus Laboratory, Virus Reference Division, Central Public Health Laboratory, Colindale, London NW9 5HT,2

3. Clinical Microbiology and Public Health Laboratory, Addenbrooke's Hospital, Cambridge CB2 2QW,3 and

4. Navrongo Health Research Centre, Navrongo, Upper East Region,4 Ghana, and

5. London School of Tropical Medicine and Hygiene, London,5 United Kingdom

Abstract

ABSTRACT An epidemiological study of rotavirus infection was conducted on specimens collected from patients with gastroenteritis and domiciled in the rural Upper Eastern Region of Ghana during 1998. Fifty isolates, randomly selected from 165 human group A rotavirus-positive samples, were G and P characterized by a reverse transcription (RT)-PCR assay using a seminested multiplex method. Rotaviruses of the G3 genotype were found to be the predominant strain (78%), followed by G2 (14%) and G1 (2%). Mixed infections, as shown by combinations of G3 and G2 (4%) and G3 and G1 (2%), were also observed. P typing showed P[4] (72.34%) to be the prevalent strain, followed by P[6] (21.3%), P[8] (2.13%), and a combination of P[4] and P[6] (4.3%).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference21 articles.

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