Affiliation:
1. Department of Natural Resources, Madison, Wisconsin 53707.
Abstract
A DNA probe, M13 mpHAW71, that detects Mycobacterium paratuberculosis in the fecal material of infected animals was developed for use in the diagnosis of Johne's disease. The probe detected as few as 10(5) M. paratuberculosis when hybridized under stringent conditions to total genomic DNA purified from bovine fecal material. When the probe was used diagnostically, it did not differentiate members of the Mycobacterium avium-M. intracellulare-M. paratuberculosis complex. Compared with culturing, the DNA probe identified 34.4% more mycobacterium-containing fecal samples, and testing took only 72 h to complete.
Publisher
American Society for Microbiology
Reference37 articles.
1. Diagnosis of progressive multifocal leukoencephalopathy by brain biopsy with biotin labeled DNA:DNA in situ hybridization;Aksamit A. J.;J. Neuropathol. Exp. Neurol.,1987
2. Deoxyribonucleic acid relatedness among Mycohaclteriun lepoae, Mvc(oba(cteriuin lepraernuriirn, and selected bacteria by dot blot and spectrophotometric deoxyribonucleic acid hybridization assays;Athwal R. D.;Int. J. Syst. Bacteriol.,1984
3. Isolation and purification of deoxyribonucleic acid from mycobacteria;Baess I.;Acta Pathol. Microbiol. Scand. Sect. B,1974
4. Deoxyribonucleic acid relatedness among species of slowly growing mycobacteria;Baess I.;Acta Pathol. Microbiol. Scand. Sect. B,1979
5. Immunological reactions caused by infection with Mvcobacfrrilm 7 paratuberculosis: a review;Bendixen P. H.;Nord. Veterinaermed.,1978
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