Detection and Identification of Actinobacillus pleuropneumoniae Serotypes 1, 2, and 8 by Multiplex PCR-Reference-Cited by-同舟云学术

Detection and Identification of Actinobacillus pleuropneumoniae Serotypes 1, 2, and 8 by Multiplex PCR

Published:2004-09 Issue:9 Volume:42 Page:4344-4348
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Schuchert Jennifer A.¹,Inzana T. J.¹,Angen Ø.²,Jessing S.²

Affiliation:

1. Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia

2. Danish Veterinary Institute, Copenhagen, Denmark

Abstract

ABSTRACT Multiplex PCR assays were developed to identify Actinobacillus pleuropneumoniae serotypes 1, 2, and 8. Primers designed for the conserved capsular polysaccharide (CP) export region amplified a 489-bp DNA fragment from all serotypes. Primers specific to the CP biosynthesis regions of serotypes 1, 2, and 8 amplified fragments of 1.6 kb, 1.7 kb, and 970 bp from only their respective serotypes.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.9.4344-4348.2004

Reference24 articles.

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