Comparison of the Real-Time PCR Method and the Gen-Probe Amplified Mycobacterium tuberculosis Direct Test for Detection of Mycobacterium tuberculosis in Pulmonary and Nonpulmonary Specimens

Author:

Lemaître Nadine1,Armand Sylvie1,Vachée Anne2,Capilliez Odile1,Dumoulin Christine1,Courcol René J.1

Affiliation:

1. Laboratoire de Bactériologie-Hygiène, Centre Hospitalier Régional Universitaire de Lille, Lille

2. Laboratoire de Biologie Polyvalente, Hôpital de Roubaix, Roubaix, France

Abstract

ABSTRACT Real-time PCR was compared to Amplified Mycobacterium tuberculosis Direct Test (AMTDII) for 100 clinical specimens. The overall sensitivities of the real-time PCR method and AMTDII were similar for respiratory and nonrespiratory specimens. However, real-time PCR seemed to be less susceptible to amplification inhibitors than AMTDII.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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