Diagnosis of acute pulmonary toxoplasmosis by visualization of invasive and intracellular tachyzoites in Giemsa-stained smears of bronchoalveolar lavage fluid

Author:

Bottone E J1

Affiliation:

1. Clinical Microbiology Laboratories, Mount Sinai Hospital, New York, New York 10029-6574.

Abstract

Two patients with AIDS presented with pulmonary toxoplasmosis. Diagnosis was achieved by the visualization, in Giemsa-stained smears of bronchoalveolar lavage fluid, of tachyzoites of Toxoplasma gondii that were extracellular and in the process of invading or within pneumocytes. The intracellular localization of tachyzoites facilitated diagnosis by obviating potential confusion of extracellular tachyzoites with cellular debris or platelets.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference12 articles.

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2. Use of the transbronchial biopsy for diagnosis of opportunistic pulmonary infections in acquired immunodeficiency syndrome (AIDS);Blumenfeld W.;Am. J. Clin. Pathol.,1984

3. Pulmonary toxoplasmosis;Catterall J. R.;Am. Rev. Respir. Dis.,1986

4. Toxoplasma gondii infection of the central nervous system. Use of the peroxidase-antiperoxidase method to demonstrate Toxoplasma in formalin fixed, paraffin embedded tissue sections;Conley F. K.;Hum. Pathol.,1981

5. Laboratory diagnosis of pulmonary toxoplasmosis in patients with acquired immunodeficiency syndrome;Derouin F.;J. Clin. Microbiol.,1989

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