Demonstration of a cell wall antigen cross-reacting with cryptococcal polysaccharide in experimental disseminated trichosporonosis

Author:

Melcher G P1,Reed K D1,Rinaldi M G1,Lee J W1,Pizzo P A1,Walsh T J1

Affiliation:

1. Department of Medicine, Wilford Hall U.S. Air Force Medical Center, Lackland Air Force Base, Texas 78236.

Abstract

Patients with disseminated infections caused by Trichosporon beigelii have a circulating antigen that cross-reacts with the polysaccharide capsule of Cryptococcus neoformans. We studied the localization of this antigen by immunoelectron microscopy in a rabbit model of experimental disseminated trichosporonosis. Deparaffinized lung sections were examined by using a murine monoclonal anti-cryptococcal polysaccharide antibody and colloidal gold particles coated with goat antibody to murine immunoglobulin G. Antigen that cross-reacted with the monoclonal antibody was observed in the T. beigelii cell wall and in a fibrillar matrix extending from the cell wall.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference17 articles.

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2. Cooper B. H. and M. Silva-Hunter. 1985. Yeasts of medical importance p. 526-541. In E. H. Lennette A. Balows W. J. Hausler. Jr.. and H. J. Shadomy (ed.). Manual of clinical microbiology. 4th ed. American Society for Microbiology Washington D.C.

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