The Cytotoxic Fimbrial Structural Subunit of Xenorhabdus nematophila Is a Pore-Forming Toxin-Reference-Cited by-同舟云学术

The Cytotoxic Fimbrial Structural Subunit of Xenorhabdus nematophila Is a Pore-Forming Toxin

Published:2006-11-15 Issue:22 Volume:188 Page:7957-7962
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Banerjee Jyotirmoy¹,Singh Jitendra²¹,Joshi Mohan Chandra²¹,Ghosh Shubhendu³,Banerjee Nirupama²

Affiliation:

1. Centre for Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India

2. International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India

3. Department of Biophysics, University of Delhi, New Delhi 110021, India

Abstract

ABSTRACT We have purified a fimbrial shaft protein (MrxA) of Xenorhabdus nematophila . The soluble monomeric protein lysed larval hemocytes of Helicoverpa armigera . Osmotic protection of the cells with polyethylene glycol suggested that the 17-kDa MrxA subunit makes pores in the target cell membrane. The internal diameter of the pores was estimated to be >2.9 nm. Electron microscopy confirmed the formation of pores by the fimbrial subunit. MrxA protein oligomerized in the presence of liposomes. Electrophysiological studies demonstrated that MrxA formed large, voltage-gated passive-diffusion channels in lipid bilayers.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00787-06

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