Influence of different conditions on kinetics of tumor necrosis factor alpha release by peripheral blood mononuclear cells after stimulation with Cryptococcus neoformans: a possible explanation for different results

Author:

Chaka W1,Verheul A F1,Hoepelman A I1

Affiliation:

1. Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, Division of Vaccines, Utrecht, The Netherlands.

Abstract

In available literature, different kinetics for tumor necrosis factor alpha (TNF-alpha) release by peripheral blood mononuclear cells are reported upon stimulation with Cryptococcus neoformans. Results in this study showed that shaking cells gives faster kinetics of TNF-alpha release while large working volumes give lower TNF-alpha concentrations. Different experimental conditions thus influence kinetics of TNF-alpha release by phagocytes.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Reference13 articles.

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2. Interleukin 10 (IL-10) inhibits the release of proinflammatory cytokines from human polymorphonuclear leucocytes. Evidence for an autocrine role of tumor necrosis factor and IL-1~ in mediating the production of IL-8 triggered by lipopolysaccharide;Cassatell M. A.;J. Exp. Med.,1993

3. .Chaka W. et al. Cytokine profiles in cerebrospinal fluid of HIV-infected patients with cryptococcal meningitis: no leucocytosis despite high IL-8 levels. J. Infect. Dis. in press.

4. Cryptococcus neoformans and cryptococcal glucuronoxylomannan, galactoxylomannan, and mannoprotein induce different levels of tumor necrosis factor alpha in human peripheral blood mononuclear cells;Chaka W.;Infect. Immun.,1991

5. Cytokine enhancement of complement-dependent phagocytosis by macrophages: synergy of TNF-~ and GMCSF for phagocytosis of Cryptococcus neoformans;Collins H. L.;Eur. J. Immunol.,1992

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