Liquid Scintillation Radioassay in Disposable Microcentrifuge Tubes: Radioimmune Precipitates and Other Applications

Author:

Schaffer F. L.1,Soergel M. E.1

Affiliation:

1. Naval Biomedical Research Laboratory, University of California, School of Public Health, Berkeley, California 94720

Abstract

A simple, economical radioassay system employing disposable polypropylene microcentrifuge tubes was developed. Plastic adapters permitted automatic operation in liquid scintillation spectrometers. Counting efficiencies of 3 H, 14 C, 32 P, and 125 I in liquid scintillation cocktails and of 32 P by Cerenkov radiation (at lower efficiency in absence of added scintillator) were comparable to those in standard vials. Multipurpose use of the microtubes made the system versatile and expedient, e.g., collection of precipitates and radioassay in the same container. Collection of radioimmune precipitates was aided by a carrier inorganic precipitate, Mg 2 P 2 O 7 .

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference15 articles.

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