Quantitative Infrared Photoanalysis of Selected Bacteria

Author:

Dugan D. L.1,Wright D. N.1

Affiliation:

1. Department of Microbiology, Brigham Young University, Provo, Utah 84602

Abstract

A technique to measure transmitted infrared radiation from minute biological systems is described. Infrared color film was exposed by radiation transmitted through bacterial colonies. The resultant photographic image was unique for each species of bacteria examined and spectral analysis of the image provided differential light emission patterns which could be quantitated. A formula for developing numerical comparisons among bacterial colonies was provided. The results of this numerical procedure gave quantitative relationships for the total infrared data from each microbial colony and made possible the differential identification of ten species of medically significant bacteria.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference9 articles.

1. Bacterial colorimetry;Bayne-Jones S.;J. Bacteriol.,1929

2. Bergey's manual of determinative bacteriology 7th ed. 1957. The Williams & Wilkins Co. Baltimore.

3. Infrared color photography: selective demonstration of bacteria;Casida L. E.;Science,1968

4. Clark W. 1946. Photography by infrared 2nd ed. John Wiley and Sons Inc. New York.

5. Engel C. E. 1968. Photography for the scientist. Academic Press Inc. New York.

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