Affiliation:
1. Department of Microbiology, The Ohio State University, Columbus, Ohio 43210
Abstract
The relative amount of extracellular polymer which remains about
Azotobacter vinelandii, Zoogloea ramigera, Klebsiella pneumoniae
, and
Diplococcus pneumoniae
after critical-point drying was studied by electron microscopy. The results obtained with this technique are compared to those obtained with methods that illustrate extracellular polymer, such as freeze-etching and ruthenium red staining. Comparative results indicate critical-point drying to be a rapid, reliable method for the determination of capsule-like polymer surrounding bacterial cells. In addition, critical-point drying can be used to observe morphogenetic changes, such as vesicle production.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Cited by
4 articles.
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