Affiliation:
1. New York State Institute for Research in Mental Retardation, Staten Island, New York 10314
Abstract
Immunization of New Zealand White rabbits with purified visna virus elicited antibody activity demonstrated by passive hemagglutination (PHA), complement fixation (CF), and indirect immunofluorescent tests. The antibody activities of hyperimmune sera and Sephadex G-200 fractions of the sera were studied. It was found that the PHA test was 10 to 100 times more sensitive than the CF test in detecting visna antibodies in rabbits. It was also found that the immunoglobulin M fractions from Sephadex G-200 filtration displayed greater PHA activity than did the immunoglobulin G fractions. Although neutralizing antibody was demonstrated in the serum of the natural host (sheep), our attempts to demonstrate neutralizing antibody in the sera from hyperimmunized rabbits (non-natural host) so far have failed.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference12 articles.
1. Complementfixing antibodies in sera of sheep affected with visna and maedi;Gudnadottir M.;J. Immunol.,1967
2. Immunofluorescence and cytochemical studies of visna virus in cell culture;Harter D. H.;J. Virol.,1967
3. Application of passive hemagglutination methods for detection of antibody to viral antigen in hamsters bearing adenovirus type 12 tumors;Lefkowitz S. S.;J. Immunol.,1968
4. Ribonucleic acid-dependent deoxyribonucleic acid polymerase in visna virus;Lin F. H.;J. Virol.,1970
5. Characterization of ribonucleic acid from visna virus;Lin F. H.;J. Virol.,1971
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