Affiliation:
1. Microbial Diseases Laboratory, California State Department of Health Services, Berkeley 94704.
Abstract
The differential and selective sodium dodecyl sulfate-polymyxin B-sucrose medium (SPS) of Kitaura et al. (T. Kitaura, S. Doke, I. Azuma, M. Imaida, K. Miyano, K. Harada, and E. Yabuuchii, FEMS Microbiol. Lett. 17:205-209, 1983), which highlights alkylsulfatase activity, was evaluated for its potential use in the direct isolation and enumeration of Vibrio vulnificus from shellfish. V. vulnificus was detected by this method in six of nine shellfish samples collected from diverse geographic locales during the summer of 1986. Direct enumeration of V. vulnificus at 7.0 X 10(2) to 2.2 X 10(4) CFU/g of shellfish was achieved on SPS agar. All sample results were confirmed in parallel examinations by using conventional glucose-salt-Teepol (Shell Oil Co.) broth and alkaline peptone water enrichment with plating onto thiosulfate-citrate-bile salts-sucrose agar. Additionally, alkylsulfatase activity was evaluated in vitro for 97 strains representing 14 Vibrio spp. V. vulnificus and Vibrio cholerae-01 were the only species consistently found to possess this activity. The range of plating efficiencies for random V. vulnificus strains analyzed on SPS was 11 to 74% (mean, 39%). The use of SPS shows great promise for the study of shellfish and other environmental sources for V. vulnificus.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference15 articles.
1. American Public Health Association. 1985. Estimation of bacterial density p. 882. In Arnold E. Greenberg R. R. Trussell and L. S. Clesceri (ed.) Standard methods for the examination of water and wastewater 16th ed. American Public Health Association Washington D.C.
2. Disease caused by a marine vibrio: clinical characteristics and epidemiology;Blake P. A.;N. Engl. J. Med.,1979
3. Spectrum of vibrio infections in a Gulf Coast community;Bonner J. R.;Ann. Intern. Med.,1985
4. Farmer J. J. III F. W. Hickman-Brenner and M. T. Kelly. 1985. Vibrio p. 282-301. In E. H. Lennette A. Balows W. J. Hausler Jr. and H. J. Shadomy (ed.) Manual of clinical microbiology 4th ed. American Society for Microbiology Washington D.C.
5. Halophilic Vibrio species isolated from blood cultures;Hollis D. G.;J. Clin. Microbiol.,1976
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