Induction and Characterization of Penicillium caseicolum Mutants Resistant to Ergosterol Biosynthesis Inhibitors

Author:

de Falandre Alexandrine1,Bouvier-Fourcade Isabelle1,Seng Jean-Marc1,Leroux Pierre1

Affiliation:

1. Laboratoire de Cryptogamie, Université de Paris-Sud, Centre d'Orsay, Bâtiment 400, 91405 Orsay Cedex, and Laboratoire de Phytopharmacie, Institut National de la Recherche Agronomique, 78000 Versailles, 2 France

Abstract

The isolation of Penicillium caseicolum mutants resistant to different fungicides which inhibit ergosterol biosynthesis is reported. Mutational frequencies for resistance were high (3 × 10 −3 to 3 × 10 −5 ). The levels of resistance toward the inhibitors of sterol C-14 demethylation were always low (<10), whereas high values were obtained with mutants resistant to inhibitors of sterol Δ14 reduction or Δ8→Δ7 isomerization, or both. Generally, there was a positive cross-resistance between fungicides showing the same biochemical mode of action but not between compounds of two different groups. Mycelial growth rate and sporulation were tested; several mutants were not affected for these characteristics. We conclude that resistance to ergosterol biosynthesis inhibitors may be used as a good marker for genetic studies through protoplast fusion.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference17 articles.

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4. Etude du r61e des microorganismes et des enzymes au cours de la maturation des fromages. III. Influence des microorganismes;Desmazeaud M. J.;Lait,1976

5. Laboratory resistance to fungicides which inhibit ergosterol biosynthesis in Penicillium italicum;de Waard M. A.;Neth. J. Plant. Pathol.,1982

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